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Easier detection of invertebrate "identification-key characters" with light of different wavelengths ArchiMer
Koken, Marcel; Grall, Jacques.
The marine alpha-taxonomist often encounters two problems. Firstly, the "environmental dirt" that is frequently present on the specimens and secondly the difficulty in distinguishing key-features due to the uniform colours which fixed animals often adopt. Here we show that illuminating animals with deep-blue or ultraviolet light instead of the normal white-light abrogates both difficulties; dirt disappears and important details become clearly visible. This light regime has also two other advantages. It allows easy detection of very small, normally invisible, animals (0.1 mu m range). And as these light wavelengths can induce fluorescence, new identification markers may be discovered by this approach.
Tipo: Text Palavras-chave: Fluorescent colours; Light emission; Determination; Cryptic species; Dimorphism; Pattern UV light; Deep-blue light; Autofluorescence.
Ano: 2011 URL: http://archimer.ifremer.fr/doc/00185/29660/28026.pdf
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RNO. Imposex-TBT. Intensité de l’imposex chez Nucella lapillus le long des côtes de la Manche et de l’Atlantique en 2009. Avril 2010 ArchiMer
Huet, Martial; Koken, Marcel.
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Ano: 2010 URL: http://archimer.ifremer.fr/doc/00019/13002/9978.pdf
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Several strains, one disease: experimental investigation of Vibrio aestuarianus infection parameters in the Pacific oyster, Crassostrea gigas ArchiMer
Travers, Marie-agnes; Tourbiez, Delphine; Parizadeh, Leila; Haffner, Philippe; Kozic-djellouli, Angelique; Aboubaker, Mohamed; Koken, Marcel; Degremont, Lionel; Lupo, Coralie.
This study investigated oyster infection dynamics by different strains of Vibrio aestuarianus isolated before and after the apparent re-emergence of this pathogen observed in France in 2011. We conducted experiments to compare minimal infective dose, lethal dose 50 and bacterial shedding for six V. aestuarianus strains. Whatever the strain used, mortality was induced in juvenile oysters by intramuscular injection and reached 90–100% of mortality within 5 days. Moreover, bacterial shedding was comparable among strains and reached its maximum after 20 h (≈10 EXP5 bacteria/mL/animal). Similarly, our first estimations of lethal dose 50 were comparable among strains (minimal infective dose around 0.4 × 10EXP5 bacteria/mL and LD50 around 10EXP5 bacteria/mL) by...
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Ano: 2017 URL: https://archimer.ifremer.fr/doc/00386/49768/50308.pdf
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